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Thermo-Mechanical, Osteoblastic Cell Growth and Attachment Behavior of Elecrospun Poly(D, L-lactide-co-glycolide) Nano-Fibers: In Vitro Study

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In the present study, Poly(D,L-lactide-co-glycolide) (PLGA) nanofibrous scaffolds were synthesized by electrospinning for soft tissue replacement at a voltage of 20 kV and polymer solution feed rate of 1 mL/h. These were characterized using various morphological, elemental and other analytical techniques. The SEM results indicated that the as-spun nanofibers are uniform, smooth and bead-free over its length. The in-vitro degradation of PLGA showed that the Proteinase K has a great effect on PLGA degradation rate. The PLGA nanofibers thermal behavior indicated that the PLGA has glass transition temperature (T g) around 50 °C with no melting point. The tensile DMA results showed a strong dependence of the viscoelastic behavior of PLGA scaffolds on testing frequency, where the storage modulus (E′) increased with increasing the testing frequency while the loss modulus (E′′) decreased. The DMA results also showed that E′′ decreased dramatically with increasing the testing temperature where its value increased up to the T g. The Bone marrow mesenchymal stem cells results showed that the cell proliferation and viability was high on the PLGA scaffold. After a short duration, the proliferation was increasing and it almost reached the same level as cells grown in the tissue culture plate. Initially, the cells toke some time to attach to the scaffold, however after some time they recovered and grew at the same rate as cells on the control surface. In conclusion, the PLGA scaffolds used in this study constitute a promising material for culturing these types of cells and for tissue engineering.
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Keywords: DIFFERENTIATION; DSC; LOSS MODULUS; PLGA NANOFIBERS; STEM CELLS; STORAGE; TGA

Document Type: Research Article

Publication date: February 1, 2015

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  • Science of Advanced Materials (SAM) is an interdisciplinary peer-reviewed journal consolidating research activities in all aspects of advanced materials in the fields of science, engineering and medicine into a single and unique reference source. SAM provides the means for materials scientists, chemists, physicists, biologists, engineers, ceramicists, metallurgists, theoreticians and technocrats to publish original research articles as reviews with author's photo and short biography, full research articles and communications of important new scientific and technological findings, encompassing the fundamental and applied research in all latest aspects of advanced materials.
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