Adenosine-5′-Triphosphate Aptamer Containing Triple-Helix DNA Capped Mesoporous Silica Nanoparticles for Controlled Release
Aptamer is an attractive material for the design of stimuli-responsive delivery systems owing to their conformational polymorphism, good biocompatibility and high affinity and specificity for binding a wide range of non-nucleic acid targets, from small molecules to whole cells. Herein, we reported a novel adenosine-5′-triphosphate (ATP) aptamer-based mesoporous silica nanoparticle (MSN) controlled release system which was based on an ATP aptamer containing triple-helix DNA (apt-THDNA) gate. In this system, the apt-THDNA gate was composed of an ATP aptamer sequence flanked by two arm segments and an alkyne modified oligonucleotide serving as a nail to be tethered onto the azide group modified MSN surface. In the absence of ATP, the apt-THDNA was capped on the MSN through hybridization of two arm segments of the ATP aptamer with the nail DNA, resulting in blockage of pores and inhibition of guest molecules release. If ATP was present, the aptamer/ATP complex was formed and got away from the pore, leaving the open of pore and release of encapsulated guest. As a proof-of-principle, Ru(bipy)2+ 3 was selected as a model guest molecule. The Ru(bipy)2+ 3-loaded system had good capping efficiency and excellent ATP response behavior. The Ru(bipy)2+ 3 released percentage can reach 90% after treatment with 10 mM ATP for 6 h. Moreover, the system showed high selectivity to ATP and good response to ATP which extracted from living cells. This proof of concept would provide a new way to design various target-responsive systems using other aptamer sequence to construct the apt-THDNA gates.
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Document Type: Research Article
Publication date: February 1, 2015
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