In SituPreparation of Size-Controlled Iron Oxide Nanocrystals Using Double-Hydrophilic Multiarm Hyperbranched Polymers as Nanoreactors and Their Magnetofection In Vitro
In situ preparation of size-controlled magnetic iron oxide nanocrystals (NCs) within double-hydrophilic multiarm hyperbranched polymers and their application as magnetic nonviral gene vectors for magnetofection has been proposed. The double-hydrophilic multiarm hyperbranched polymers were conveniently constructed by grafting poly(ethylene glycol) methyl ether acrylate (PEGMEA) molecules to hyperbranched poly(ethylenimine)s (HPEI) with the help of Michael addition. HPEI conjugates with 7.5, 15.7 and 23 PEGMEA grafts (referred to as HPEI-PEGMEA7.5, HPEI-PEGMEA15.7 and HPEI-PEGMEA23, respectively) were synthesized. By introducing biocompatible PEGMEA molecules to HPEI, the cytotoxicity of the resulting HPEI-PEGMEA can be greatly decreased compared with HPEI. The HPEI-PEGMEA double-hydrophilic hyperbranched polymers were then used as nanoreactors and stabilizers to prepare size-controlled magnetic iron oxide nanocrystals. With the increase of PEGMEA arms, the size and magnetization of magnetic iron oxide NCs were improved, resulting in the enhancement of gene transfection efficiency. The resulting magnetic gene vectors had magnetic targeting, low cytotoxicity and their magnetofection efficiency could reach 4.5 fold of that for standard HPEI transfection.
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Document Type: Research Article
Publication date: February 1, 2015
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