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ZnO Nanoparticles: Cytological Effect on Chick Fibroblast Cells and Antimicrobial Activities Towards Escherichia Coli and Bacillus Subtilis

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The synthesis of ZnO nanoparticles and their cytological effects on chick fibroblast cells and antimicrobial activities towards Escherichia Coli (E. Coli) and Bacillus Subtilis (B. Subtilis) are reported. The ZnO nanoparticles were synthesized by non-protonated process using p-xylene (C6H4(CH3)2 and zinc acetate di-hydrate (Zn(CH3COO)2ยท2H2O) and characterized by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray powder diffractometer (XRD), energy dispersive spectroscopy (EDS) and fourier transform infrared (FTIR) spectroscopy which confirmed the high-density growth, well-crystallinity, pure and wurtzite hexagonal phase for prepared nanoparticles. To investigate the cytological effect on chick fibroblast cells, as-synthesized ZnO nanoparticles were treated as dose dependent manner at 24 h incubation. The viability of the cells was analyzed by MTT method. At 24 h, the % cell death was found to vary from 18, 51 and 62% at 5, 50 and 100 μg/ml concentrations of ZnO nanoparticles, respectively. The reactive oxygen species (ROS) was also measured with fibroblast cells using various doses of ZnO nanoparticles and ROS was measured as 121, 189 and 210% at nanoparticle concentrations of 5, 50 and 100 μg/mL, respectively. Additionally, as-synthesized ZnO nanoparticles were also tested against pathogenic bacteria E. Coli and B. Subtilis via disk diffusion method and nanoparticles exhibited good inhibition of microbial strains.
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Keywords: BACILLUS SUBTILIS; CHICK FIBROBLAST CELLS; E. COLI; ZNO NANOPARTICLES

Document Type: Research Article

Publication date: November 1, 2013

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  • Science of Advanced Materials (SAM) is an interdisciplinary peer-reviewed journal consolidating research activities in all aspects of advanced materials in the fields of science, engineering and medicine into a single and unique reference source. SAM provides the means for materials scientists, chemists, physicists, biologists, engineers, ceramicists, metallurgists, theoreticians and technocrats to publish original research articles as reviews with author's photo and short biography, full research articles and communications of important new scientific and technological findings, encompassing the fundamental and applied research in all latest aspects of advanced materials.
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