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Antibacterial Activity of Nanocrystalline TiO2(B) on Multiresistant Klebsiella pneumoniae Strains

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Nanocrystalline TiO2(B) and bi-crystalline (anatase-TiO2(B)) photocatalysts were prepared through hydrothermal synthesis by using different Ti-precursors such as titanium(IV) isopropoxide, titanium(IV) chloride and Degussa P25 TiO2. The obtained TiO2 samples were characterized by means of X-ray diffraction, X-ray photoelectron spectroscopy, transmission electron microscopy, and N2-sorption measurements. Both the singlephase and bi-crystalline photocatalysts were composed of rod-like, polydisperse nanocrystallites as revealed by electron microscopy analysis. The antibacterial activity of the TiO2 samples was tested against three different Klebsiella pneumoniae strains in aqueous dispersions illuminated by UV-A light. We found that UV-A irradiation itself did not decrease the viable cell number of the bacteria. However, TiO2 nanorods irradiated with UV-A light exhibited high antibacterial activity even in the case of the TiO2 samples synthesized under mild reaction conditions without applying any post-annealing step. The photocatalytic activity of the samples was also tested via methyl orange degradation. We found that bi-crystalline anatase-TiO2(B) possessed higher antibacterial and photocatalytic activities than that of single-phase TiO2(B). From these nanostructures, transparent TiO2 thin films of high quality on glass substrates were successfully fabricated by dip-coating method without the use of any additives.
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Keywords: ANTIBACTERIAL ACTIVITY; HYDROTHERMAL SYNTHESIS; KLEBSIELLA PNEUMONIAE; TIO2 THIN FILMS; TIO2(B)

Document Type: Research Article

Publication date: September 1, 2013

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  • Science of Advanced Materials (SAM) is an interdisciplinary peer-reviewed journal consolidating research activities in all aspects of advanced materials in the fields of science, engineering and medicine into a single and unique reference source. SAM provides the means for materials scientists, chemists, physicists, biologists, engineers, ceramicists, metallurgists, theoreticians and technocrats to publish original research articles as reviews with author's photo and short biography, full research articles and communications of important new scientific and technological findings, encompassing the fundamental and applied research in all latest aspects of advanced materials.
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