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The Targeted Regulation of Canine Optic Nerve CB2R Affects the Direction of Microglial Cell Differentiation

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This study investigated the effect of targeted regulation of cannabinoid 2 receptor (CB2R) on the direction of microglia (MG) differentiation in the optic nerve of beagle dogs. Additionally, the promotion of differentiation of MG into M2-type by CB2R agonist was investigated. The optic nerves of beagle dogs were used to make the original generation of cell culture, and microscopy was used to observe cell morphology and immunofluorescence using IFN-gamma, AM630, and AM1241 was used to verify MG. The following four experimental groups were established: resting, inflammation, inflammation with agonist, and inflammation with antagonist groups. Enzyme-linked immunosorbent assay (ELISA) and quantitative real time polymerase chain reaction (qRT-PCR) were used to examine the regulation of CB2R, since it induces MG to differentiate into M1- or M2-type, and thus plays different roles. MG was significantly activated in the inflammatory group, and a larger proportion of M1-type microglia was observed than the M2-type. The M2 proportion was higher in the inflammation with agonist group, while the M1 proportion was significantly increased in the inflammation with antagonist group. Targeted positive regulation of CB2R in the optic nerves of beagle dogs increased MG differentiation to M2, and its targeted negative regulation increased MG differentiation to M1.
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Keywords: BEAGLE DOG; CB2R; CELL DIFFERENTIATION; MICROGLIA

Document Type: Short Communication

Publication date: June 1, 2019

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  • Nanoscience and Nanotechnology Letters (NNL) is a multidisciplinary peer-reviewed journal consolidating nanoscale research activities in all disciplines of science, engineering and medicine into a single and unique reference source. NNL provides the means for scientists, engineers, medical experts and technocrats to publish original short research articles as communications/letters of important new scientific and technological findings, encompassing the fundamental and applied research in all disciplines of the physical sciences, engineering and medicine.
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