Portable Optomagnetic Microfluidic Chip System

A highly sensitive photomagnetic microfluidic chip was designed to achieve one-step detection of low concentration substances by integrating three-dimensional chip fabrication, bidirectional magnetic micro particle technology, and total reflection imaging technique. The new device takes advantage of three functions of magnetic particles: enrichment, separation and speckle imaging. The total reflection optical magnetic imaging eliminates the use of chemiluminescent or fluorescent reagents, reducing the number of detection steps. The optimization of the electromagnetic control simulation, the microfluidic simulation analysis, and the 3D chip structure are detailed. A bidirectional converter magnetic field technology can accelerate the sandwich immune reaction with enrichment of the marker, improving the accuracy and speed of detection. Due to a high precision one-step immunoassay, the detection time is less than 5 min, and the amount of sample needed is very low (<10 μL). A series of concentrations of C-reactive protein (CRP) was used for pattern detection, and signals obtained from CRP at 0.02 to 20 ng/mL were analyzed to generate a standard calibration curve. The novel one-step multiparameter optical chip detection system is uniquely suited for rapid early diagnosis in a clinical setting, as well as for biological research applications.