Ecdysterone Promotes Osteoblast Differentiation Through ERα Mediated AMPKα Signal Inhibition and β-Catenin Signal Activation
Achyranthes bidentata is a traditional Chinese medicine used for osteoporosis treatment for many years; however, the mechanism through which it cures the disease is largely unclear. Ecdysterone is an active component of Achyranthes bidentata. We hypothesized that it played a vital role in alleviating the osteoporosis disease symptoms. Therefore, this study was conducted to investigate the underlying mechanism, which ecdysterone adopts to influence the osteoblast's functioning. The results of CCK8 assay showed that ecdysterone stimulation could increase osteoblasts viability significantly while alkaline phosphatase (ALP) assay and immunocytochemical staining also manifested increased expression of ALP, Collagen I, and osteoprotegerin upon ecdysterone incitement. These results imply that ecdysterone stimulus could promote osteoblasts differentiation. Furthermore, the RT-PCR and WB showed ecdysterone exposure could substantially upregulated the expression of ERα, instead of ERβ and ERRα. Similarly, the expression of critical molecules of osteogenetic differentiation, such as p-AMPKα and β-catenin, was also increased after ecdysteronetreatment. In order to further probe the underlying mechanisms, osteoblasts were transfected with siRNA to silence the ERα gene, and the expression of p-AMPKα, β-catenin, and osteogenetic biomarkers was recorded with or without ecdysterone treatment. These results confirmed that ecdysterone promoted osteoblasts differentiation mainly through ERα, and ERα may mediate the AMPKα inhibition and β-catenin activation in this process.
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Document Type: Short Communication
Publication date: September 1, 2017
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- Nanoscience and Nanotechnology Letters (NNL) is a multidisciplinary peer-reviewed journal consolidating nanoscale research activities in all disciplines of science, engineering and medicine into a single and unique reference source. NNL provides the means for scientists, engineers, medical experts and technocrats to publish original short research articles as communications/letters of important new scientific and technological findings, encompassing the fundamental and applied research in all disciplines of the physical sciences, engineering and medicine.
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