Skip to main content
padlock icon - secure page this page is secure

Magnetic Nanoparticles Enhanced DNA Extraction and Detection of Forensic Algae by Multiplex PCR Capillary Electrophoresis

Buy Article:

$105.00 + tax (Refund Policy)

PCR detection of drowning related algal DNA from organs of drowning victims has attracted huge interest in forensic research. Magnetic nanoparticles (MNPs) have been regarded as an efficient tool for automatic DNA extraction; however, whether the algal DNA can be efficiently extracted has remained doubtful. In this study, a multiplex PCR-Capillary electrophoresis (MPCE) system was established by using two popular algal primers for SSU and UPA genes respectively. Three types of commercially available MNPs were then used for DNA extraction from the tissues of 20 cadavers found in the water. The MNPs were evaluated by the MPCE system and results showed that the MNPs (XiaoMai Biotech) significantly increased the positive detection rate (80%) (P < 0.05) compared to others. The MNPs also had better result than the Microwave Digestion-Vacuum Filtration-Automated Scanning Electron Microscopy (MD-VF-Auto SEM) test method (75%, P > 0.05).
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: CAPILLARY ELECTROPHORESIS; DIATOM; DROWNING; FORENSIC MEDICINE; MAGNETIC NANOPARTICLE

Document Type: Short Communication

Publication date: August 1, 2016

More about this publication?
  • Nanoscience and Nanotechnology Letters (NNL) is a multidisciplinary peer-reviewed journal consolidating nanoscale research activities in all disciplines of science, engineering and medicine into a single and unique reference source. NNL provides the means for scientists, engineers, medical experts and technocrats to publish original short research articles as communications/letters of important new scientific and technological findings, encompassing the fundamental and applied research in all disciplines of the physical sciences, engineering and medicine.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more