Magnetic Nanoparticles Enhanced DNA Extraction and Detection of Forensic Algae by Multiplex PCR Capillary Electrophoresis
PCR detection of drowning related algal DNA from organs of drowning victims has attracted huge interest in forensic research. Magnetic nanoparticles (MNPs) have been regarded as an efficient tool for automatic DNA extraction; however, whether the algal DNA can be efficiently extracted has remained doubtful. In this study, a multiplex PCR-Capillary electrophoresis (MPCE) system was established by using two popular algal primers for SSU and UPA genes respectively. Three types of commercially available MNPs were then used for DNA extraction from the tissues of 20 cadavers found in the water. The MNPs were evaluated by the MPCE system and results showed that the MNPs (XiaoMai Biotech) significantly increased the positive detection rate (80%) (P < 0.05) compared to others. The MNPs also had better result than the Microwave Digestion-Vacuum Filtration-Automated Scanning Electron Microscopy (MD-VF-Auto SEM) test method (75%, P > 0.05).
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Document Type: Short Communication
Publication date: August 1, 2016
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- Nanoscience and Nanotechnology Letters (NNL) is a multidisciplinary peer-reviewed journal consolidating nanoscale research activities in all disciplines of science, engineering and medicine into a single and unique reference source. NNL provides the means for scientists, engineers, medical experts and technocrats to publish original short research articles as communications/letters of important new scientific and technological findings, encompassing the fundamental and applied research in all disciplines of the physical sciences, engineering and medicine.
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