Immobilization of Lipase Onto Dopamine Functionalized Magnetic Nanoparticles
A nanocarrier Fe3O4 @ OA @ DA, with the structure of a dopamine functionalized Fe3O4 nanoparticles coated with oleic acid (OA), was simply prepared by dipping the Fe3O4 @ OA magnetic nanoparticles in dopamine solution. Lipase from Thermomyces lanuginosus was immobilized onto the obtained nanocarrier via covalent coupling. A high immobilization efficiency of 88%, and an immobilization yield of 97% with enzyme loading of 13.09 mg/g-support were achieved under the optimal conditions: immobilization time 10 h, pH 7.0, and enzyme concentration 0.27 mg/mL. Compared with the free enzyme, thermostability of the immobilized lipase was 2.9-fold improved at 60 °C. More than 75% initial activity of the immobilized lipase was remained after ten repeated uses. The V max and Km of the free and immobilized lipase were determined as 2.51 mmol/min, 2.13 mmol/min and 8.86 mM, 10.50 mM, respectively. These results indicated that the prepared magnetic oleic acid nanoparticle with dopamine functionalization is a good carrier for lipase immobilization, and has the potential uses for immobilization of other enzymes.
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Document Type: Short Communication
Publication date: March 1, 2016
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- Nanoscience and Nanotechnology Letters (NNL) is a multidisciplinary peer-reviewed journal consolidating nanoscale research activities in all disciplines of science, engineering and medicine into a single and unique reference source. NNL provides the means for scientists, engineers, medical experts and technocrats to publish original short research articles as communications/letters of important new scientific and technological findings, encompassing the fundamental and applied research in all disciplines of the physical sciences, engineering and medicine.
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