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Investigation of Interaction Between Casein Enzyme Hydrolysate and Biosynthesized Silver Nanoparticles by Spectroscopy

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The interaction between silver nanoparticles and casein enzyme hydrolysate-Type I have been carried out by spectroscopic techniques (fluorescence, UV-Visible, and circular dichroism) at different temperatures (293, 303 and 313 K). UV-Vis spectroscopic data suggested the formation of ground state complex between casein enzyme hydrolysate and silver nanoparticles. The silver nanoparticles have a strong ability to quench the intrinsic fluorescence of casein enzyme hydrolysate by quenching mechanisms through dynamic quenching process. The number of binding sites 'n' and binding constants 'K' were determined at different temperatures based on fluorescence quenching. The thermodynamic parameters (ΔH, ΔG, and ΔS) of the interaction system were calculated at different temperatures and the results suggested that the binding process was spontaneous and the acting force between casein enzyme hydrolysate and silver nanoparticles were mainly hydrophobic forces and electrostatic interaction. Based on the Förster non-radiation energy transfer theory, the average binding distance between casein enzyme hydrolysate and silver nanoparticles was estimated to be 4.67 nm. Both synchronous fluorescence and circular dichroism spectra confirmed the interaction as mentioned which reflected in the conformational change of casein enzyme in presence of silver nanoparticles.
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Document Type: Short Communication

Publication date: July 1, 2014

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  • Nanoscience and Nanotechnology Letters (NNL) is a multidisciplinary peer-reviewed journal consolidating nanoscale research activities in all disciplines of science, engineering and medicine into a single and unique reference source. NNL provides the means for scientists, engineers, medical experts and technocrats to publish original short research articles as communications/letters of important new scientific and technological findings, encompassing the fundamental and applied research in all disciplines of the physical sciences, engineering and medicine.
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