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Enhanced Resonance Light Scattering of Antibody Covalently Conjugated Gold Nanoparticles Due to Antigen-Antibody Interaction Induced Aggregation

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Antibody coated nanoparticles have been used extensively in optical immunoassay for detecting various proteins. However, in most cases, the strategies for preparing antibody attached nanoparticles were based on physical adsorption, which is adverse to promote the detection limit and sensitivity of optical immunoassay. In this paper, antibody was covalently conjugated to gold nanospheres using a long chain alkanethiol: 16-mercaptohexadecanoic acid (16-MHDA). 16-MHDA modified gold nanoparticles were more stable and easier to conjugate antibody. Characterized results confirmed that antibody was covalently coated on the surface of gold nanoparticles. Owing to antigen-antibody interaction, the fabricated gold nanoparticles/antibody bioconjugates produced specific aggregation in the presence of antigen, which enhanced the resonance light scattering intensity. The enhanced intensity was highly sensitive to the concentration of antigen. The results suggested that such bioconjugates had great potential in detecting proteins using resonance light scattering with high sensitivity.
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Keywords: ALPHA-FETOPROTEIN (AFP); ANTIBODY CONJUGATED NANOPARTICLES; GOLD NANOPARTICLES (AU NPS); RESONANCE LIGHT SCATTERING (RLS)

Document Type: Research Article

Publication date: August 1, 2013

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  • Nanoscience and Nanotechnology Letters (NNL) is a multidisciplinary peer-reviewed journal consolidating nanoscale research activities in all disciplines of science, engineering and medicine into a single and unique reference source. NNL provides the means for scientists, engineers, medical experts and technocrats to publish original short research articles as communications/letters of important new scientific and technological findings, encompassing the fundamental and applied research in all disciplines of the physical sciences, engineering and medicine.
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