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Open Access Effect of rat bone marrow stromal stem cells overexpressing platelet-derived growth factor and bone morphogenic protein into platelet-rich plasma gels on osteogenic differentiation

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The aim of this study was to improve osteoblast function by incorporating rat bone marrow stromal stem cells (rBMSCs) overexpressing platelet-derived growth factor (PDGF-BB) and bone morphogenic protein (BMP-2) into platelet-rich plasma (PRP) gels. rBMSCs were isolated, cultured, and identified. The rBMSCs were subsequently co-transfected with two recombinant adenoviruses delivering PDGF-BB-GFP and BMP-2-GFP. PDGF-BB and BMP-2 expression levels in transduced BMSCs were detected, and a post-transfection analysis of the osteogenic differentiation trend of rBMSCs was performed. Autologous PRP gels were constructed and optimized, and the levels of growth factor in PRP were detected. The optimal growth conditions of the genetically-modified rBMSCs in the scaffolds were established, and the effects of tissue engineering materials and PRP gel construction on the osteogenic differentiation of rBMSCs were assessed. The results revealed that high-purity rBMSCs were obtained, and high levels of BMP-2 and PDGF-BB were secreted by the transduced cells. Furthermore, PRP promoted the proliferation and osteogenic differentiation of rBMSCs overexpressing PDGF-BB and BMP-2. Collectively, the results of the present study revealed that genetically modified rBMSCs incorporated into PRP gels enhanced osteogenic differentiation.

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Keywords: BONE MORPHOGENETIC PROTEIN; BONE REGENERATION; OSTEOBLAST; OSTEOINTEGRATION; PLATELET-DERIVED GROWTH FACTOR; PLATELET-RICH PLASMA

Document Type: Research Article

Publication date: July 1, 2020

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  • Materials Express is a peer-reviewed multidisciplinary journal reporting emerging researches on materials science, engineering, technology and biology. Cutting-edge researches on the synthesis, characterization, properties, and applications of a very wide range of materials are covered for broad readership; from physical sciences to life sciences. In particular, the journal aims to report advanced materials with interesting electronic, magnetic, optical, mechanical and catalytic properties for industrial applications.
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