Exosomal microRNAs (miRNAs) have attracted increasing interest as biomarkers for the diagnosis of numerous human diseases; however, little is known about exosomal miRNAs in regard to pediatric obstructive sleep apnea syndrome (OSAS). The aim of this study was to identify exosomal miRNAs
involved in OSAS and to determine their relative functions. Serum exosomal miRNA-expression patterns in pediatric OSAS patients and healthy donors were analyzed comprehensively via RNA sequencing, and differently expressed miRNAs were verified using quantitative reverse transcription polymerase
chain reaction. The effect of the miRNAs on cell culture was measured by flow cytometry. Results revealed that 364 and 464 miRNAs were identified in normal and OSAS exosomes, respectively. Moreover, exosomal miR-664a-3p, miR-210, miR-21-3p, and miR-107 were significantly more downregulated
in exosomes from OSAS than in those of healthy controls. These downregulated miRNAs were mainly involved in functions involving cell cycle, immune response, and cell proliferation and adhesion as well as pathways associated with mitogen-activated protein kinase, Wnt, and mammalian target rapamycin
signaling. Furthermore, miR-107 promoted the arrest of the adenoid lymphocyte cycle at the G2/M phase. These data revealed several potential exosomal miRNA biomarkers and their associated functions and pathways. This study advanced the knowledge of OSAS exosome biology to facilitate the development
of OSAS-specific exosome-based diagnostics and therapeutics.
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Obstructive Sleep Apnea Syndrome;
Document Type: Research Article
Clinical Laboratory Center, Children’s Hospital of Fudan University, Shanghai 201102, PR China
Department of Otolaryngology, Affiliated Eye and ENT Hospital of Fudan University, Shanghai 200031, PR China
Cardiovascular Center, Children’s Hospital of Fudan University, Shanghai 201102, PR China
March 1, 2020
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