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Open Access Study on the anti-inflammatory and lipid-lowering activities of novel chimeric peptide

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Acne was treated by combining the N-terminal of HPA3NT3 (where Lys in position 13 was replaced by Asn) and the C-terminal antibacterial peptide HAG of GLP-1 (32–36) amide pentapeptide after amino replacement. The minimal inhibitory concentration (MIC) was measured using the broth dilution method to evaluate HAG’s antibacterial activity. HAG’s cytotoxicity was determined using water-soluble tetrazolium salt (WST-1). Interleukin-8 (IL-8) expression in human immortalized keratinocytes (HaCaT) cells stimulated by Propionibacterium acnes and treated with HAG was measured by Enzyme-linked immunosorbent assay (ELISA). IL-8 and toll-like receptor 2 (TLR2) expression was detected using real-time reverse transcriptase polymerase chain reaction (qRTPCR) to analyze HAG’s anti-inflammatory effect in vitro. The total RNA was extracted using SiO2 nanoparticles. Oil red O staining was used to detect the intracellular lipid drop of Sebaceous Gland Cell Line (SZ95), and ELISA was used to detect triglyceride levels. Hematoxylin-eosin (HE) staining was used to evaluate ear edema induced by Propionibacterium acne in mice. The MIC of HAG in Propionibacterium acnes was 6.3 μg/mL, and 50 μg/mL of HAG showed cytotoxicity. HAG significantly reduced TLR2 and IL-8 expression in HaCaT cells. Oil red O staining showed that the lipid distribution of HAG-treated SZ95 and triglyceride secretion decreased. HAG reduced ear swelling induced by Propionibacterium acnes. HAG has anti-bacterial and anti-inflammatory effects, excellent hypolipidemic function, and low cytotoxicity. Further development of HAG could be a promising and effective reagent for acne therapy.
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Keywords: Anti-Inflammatory; Lipid-Lowering; Novel Chimeric Peptide-HAG; Propionibacterium acnes

Document Type: Research Article

Affiliations: 1: Department of Medical Aesthetics, Guangdong Provincial Maternal and Child Health Hospital, Guangzhou 510010, Guangdong, PR China 2: State Key Laboratory of Respiratory Disease, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital, The Sixth Affiliated Hospital, Department of Biomedical Engineering, Guangzhou Medical University, Guangzhou 511436, Guangdong, PR China

Publication date: March 1, 2020

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  • Materials Express is a peer-reviewed multidisciplinary journal reporting emerging researches on materials science, engineering, technology and biology. Cutting-edge researches on the synthesis, characterization, properties, and applications of a very wide range of materials are covered for broad readership; from physical sciences to life sciences. In particular, the journal aims to report advanced materials with interesting electronic, magnetic, optical, mechanical and catalytic properties for industrial applications.
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