Skip to main content
padlock icon - secure page this page is secure

Open Access Effect of smoking on colonization by Bacteroides forsythus in patients with chronic periodontitis

Download Article:
(PDF 3,899.9 kb)
Bacteroides forsythus is the least investigated species of the "red complex" of bacteria responsible for periodontitis. This is due, at least in part, to the difficulty in identifying these bacteria by classic techniques requiring in vitro cultures. Thus, the aim of the current study was to design a fast and robust technique for the detection of B. forsythus. For this purpose, DNA was isolated by magnetic microbeads from dental plaque and gingival crevical fluid, and the presence of the gene coding for 16S rRNA of B. forsythus was detected by conventional of quantitative real-time PCR. The study enrolled patients affected by periodontitis and healthy subjects, and both groups were comprised of heavy and mild smokers. The assays revealed that bot gingival fluid and dental plaque served well as the material for test samples. The periodontitis group had a markedly higher rate of infection by B. forsythus than healthy subjects. The infection rate was also higher in the group of smokers but did not depend on whether the patients were heavy or mild smokers. In conclusion, the novel methodology may become a powerful tool in the hands of clinicians, allowing them to obtain a faster and more accurate diagnosis of B. forsythus in periodontal infections.

31 References.

No Supplementary Data.
No Article Media
No Metrics


Document Type: Research Article

Publication date: August 1, 2019

More about this publication?
  • Materials Express is a peer-reviewed multidisciplinary journal reporting emerging researches on materials science, engineering, technology and biology. Cutting-edge researches on the synthesis, characterization, properties, and applications of a very wide range of materials are covered for broad readership; from physical sciences to life sciences. In particular, the journal aims to report advanced materials with interesting electronic, magnetic, optical, mechanical and catalytic properties for industrial applications.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more