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Biosynthesis of Silver Nanoparticles Using Salix viminalis and Screening Its Antibacterial Activity

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An eco-friendly method of green mediated synthesis of metallic nanoparticles is important step in the field of nanotechnology. In this study we have investigated biosynthesis of silver nanoparticles using a mixture of Salix viminalis leaves extract as a reducing agent and surfactant (CTAB) as stabilizer. These biosynthesized nanoparticles characterized with the help of UV-vis spectrophotometer, DLS, FTIR, and TEM. Nanoparticle size distribution was determined within 1 to 100 nm by TEM and DLS and the optimum wavelength was recorded in 400 to 450 nm by UV-vis spectroscopic studies. The chemical groups studies using FT-IR analysis. FT-IR spectrum indicates that the nanoparticles are bound to protein through the carboxylate ion group. In this study we observed that S. viminalis leaf extract reduce silver ion in to silver nanoparticles within 9 mins of reaction time which showed that it is a rapid synthesis. These synthesized silver nanoparticles showed good antimicrobial properties against both pathogens E. coli (gram negative) and S. aureus (gram positive) with the maximum 10 mm inhibition zone.

Keywords: ANTIMICROBIAL ACTIVITY; DLS; FT-IR; S. VIMINALIS LEAVES; SILVER NANOPARTICLES; TEM; UV

Document Type: Research Article

Publication date: 01 June 2016

More about this publication?
  • Bionanoscience attempts to harness various functions of biological macromolecules and integrate them with engineering for technological applications. It is based on a bottom-up approach and encompasses structural biology, biomacromolecular engineering, material science, and engineering, extending the horizon of material science. The journal aims at publication of (i) Letters (ii) Reviews (3) Concepts (4) Rapid communications (5) Research papers (6) Book reviews (7) Conference announcements in the interface between chemistry, physics, biology, material science, and technology. The use of biological macromolecules as sensors, biomaterials, information storage devices, biomolecular arrays, molecular machines is significantly increasing. The traditional disciplines of chemistry, physics, and biology are overlapping and coalescing with nanoscale science and technology. Currently research in this area is scattered in different journals and this journal seeks to bring them under a single umbrella to ensure highest quality peer-reviewed research for rapid dissemination in areas that are in the forefront of science and technology which is witnessing phenomenal and accelerated growth.
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