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Using Natural Plant Exudate to Separate Gold Nanoparticles Using Density Gradient Centrifugation

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An economical method of gold nanoparticles (GNPs) separation using A. indica gum, a natural plant exudate rich in carbohydrates is presented in this communication. A density gradient made up of A. indica gum in different concentrations (10–50%) was layered followed by 1 ml solution of GNPs made using A. indica leaf extract. UV-Vis spectra of GNPs made using A. indica leaf displayed a dual peak at 530 and 718 nm. Dual peaks are characteristics of anisotropic GNPs which was further confirmed by Scanning Electron Microscopy (SEM). After centrifugation for 50 minutes at 4000 rpm, distinct zones were obtained all exhibiting unique spectral properties and morphologies. To validate the results, mixture of gold nano-rods and spherical nanoparticles were centrifuged using the similar method. Results obtained verified the separation efficiency of A. indica gum.
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Keywords: A. INDICA GUM; ANISOTROPIC GOLD NANOPARTICLES; DENSITY GRADIENT; GOLD NANO-RODS

Document Type: Research Article

Publication date: August 1, 2013

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  • Bionanoscience attempts to harness various functions of biological macromolecules and integrate them with engineering for technological applications. It is based on a bottom-up approach and encompasses structural biology, biomacromolecular engineering, material science, and engineering, extending the horizon of material science. The journal aims at publication of (i) Letters (ii) Reviews (3) Concepts (4) Rapid communications (5) Research papers (6) Book reviews (7) Conference announcements in the interface between chemistry, physics, biology, material science, and technology. The use of biological macromolecules as sensors, biomaterials, information storage devices, biomolecular arrays, molecular machines is significantly increasing. The traditional disciplines of chemistry, physics, and biology are overlapping and coalescing with nanoscale science and technology. Currently research in this area is scattered in different journals and this journal seeks to bring them under a single umbrella to ensure highest quality peer-reviewed research for rapid dissemination in areas that are in the forefront of science and technology which is witnessing phenomenal and accelerated growth.
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