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Green Synthesis of Silver Nanoparticles (SNPs) Using Aegle marmelos Linn. and Its Antibacterial Potential

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Among the methods involved in synthesis of metal nanoparticals, biological methods of synthesis are currently gaining importance and reliable due to its cost effective, eco-friendly and usage of non-toxic materials in the processing. Therefore, the present study is focused on the synthesis of silver nanoparticles (SNPs) with leaf extract of holy plant Aegle marmelos Linn. by using 1 mM silver nitrate (AgNO3) solution. From the bio-reduction reaction mixture, the formation of SNPs was studied by UV-VIS spectrophotometer at the range of 390 nm to 500 nm and found maximum absorbance at 440 nm. The dried SNPs were characterized by using Transmission Electron Microscope (TEM) to observe the size, shape and distribution of particles and analyzed for confirmation of silver in SNPs by X-ray Diffraction (XRD) crystallographic method. Further, the SNPs were anlaysed for the protein or organic group by Fourier Transformed Infra Red (FTIR) spectrophotometer and found the peak of absorption at 500 to 700 nm. Finally, the antibacterial activity of SNPs were tested against few pathogenic and nonpathogenic bacteria like E.coli, Vibrio cholereae, Proteus vulgaris, Psuedomonas aurugenosa and Bacillus licheniformis by using standard methods and the results indicates that the SNPs alone treated has inhibited the bacteria at mild to moderate level in E.coli and Vibrio cholerae . However, the SNPs mixed with antibiotics inhibited the growth of all the species of bacteria tested. From the present study, it was concluded that the plant leaf extracts from Aegle marmelos Linn. can be used as a bio-reductant for the synthesis of SNPs which is environmental friendly and cost effective method. By standardizing the biosynthesis method, SNPs can be prepared in bulk with safe process environment and it can be used for various medical applications including control of pathogenic microorganisms.
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Keywords: AEGLE MARMELOS; ANTIBACTERIAL TESTING; BIO-REDUCTION; GREEN SYNTHESIS; PLANT EXTRACTS; SILVER NANOPARTICLES

Document Type: Research Article

Publication date: August 1, 2013

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  • Bionanoscience attempts to harness various functions of biological macromolecules and integrate them with engineering for technological applications. It is based on a bottom-up approach and encompasses structural biology, biomacromolecular engineering, material science, and engineering, extending the horizon of material science. The journal aims at publication of (i) Letters (ii) Reviews (3) Concepts (4) Rapid communications (5) Research papers (6) Book reviews (7) Conference announcements in the interface between chemistry, physics, biology, material science, and technology. The use of biological macromolecules as sensors, biomaterials, information storage devices, biomolecular arrays, molecular machines is significantly increasing. The traditional disciplines of chemistry, physics, and biology are overlapping and coalescing with nanoscale science and technology. Currently research in this area is scattered in different journals and this journal seeks to bring them under a single umbrella to ensure highest quality peer-reviewed research for rapid dissemination in areas that are in the forefront of science and technology which is witnessing phenomenal and accelerated growth.
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