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Synthesis of Supra-Stable Gold Nanoparticles and Size Dependent Separation Using Azadirachta indica Gum: A Green Alternative to Density Gradient Centrifugation

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We report the novel biological method for the synthesis of environmentally benign nano-particles and its size dependent seperation using natural plant exudate of Azadirachta indica (Neem) gum as density gradient centrifugation. Three distinct layers were seen after centrifugation at 4000 rpm for 70 minutes. Spectral properties of each fraction revealed the morphological features of Gold nanoparticles (GNPs). Further studies of the morphology were done using Scanning Electron Microscopy. Supra-stable nanoparticles owing to negligible red shift after addition of approximately 18 M salt were observed in the solution after reduction of the gold ions at 100 °C. Also, Flocculation Parameter was found to increase with respect to the salt concentration. Less flocculation parameter is an indication of enhanced stability of the nanoparticles. Biocompatibility of the GNPs was studied using Mardin-Darby Canine Kidney cells (MDCK) cells and found to be extremely biocompatible.
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Keywords: A. INDICA GUM; DENSITY GRADIENT CENTRIFUGATION; FLOCCULATION PARAMETER; MDCK CELLS; SUPRA-STABLE

Document Type: Research Article

Publication date: August 1, 2013

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  • Bionanoscience attempts to harness various functions of biological macromolecules and integrate them with engineering for technological applications. It is based on a bottom-up approach and encompasses structural biology, biomacromolecular engineering, material science, and engineering, extending the horizon of material science. The journal aims at publication of (i) Letters (ii) Reviews (3) Concepts (4) Rapid communications (5) Research papers (6) Book reviews (7) Conference announcements in the interface between chemistry, physics, biology, material science, and technology. The use of biological macromolecules as sensors, biomaterials, information storage devices, biomolecular arrays, molecular machines is significantly increasing. The traditional disciplines of chemistry, physics, and biology are overlapping and coalescing with nanoscale science and technology. Currently research in this area is scattered in different journals and this journal seeks to bring them under a single umbrella to ensure highest quality peer-reviewed research for rapid dissemination in areas that are in the forefront of science and technology which is witnessing phenomenal and accelerated growth.
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