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Preparation of Supramolecular λ-Deoxyribose Nucleic Acid and, Multi-Walled Carbon Nanotube Bioconjugate (λ-DNA-MW-CNT) Towards Prototype Biosensor Development

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We report Multi Walled Carbon Nanotubes (MW-CNTs) functionalization and, Deoxyribose Nucleic Acid (DNA) conjugate preparation (MW-CNT-DNA) by a simple process. The mixed chirality, multi-walled carbon nanotubes were dispersed in 1% (v/v) aqueous Triton-X and, chemically functionalized through nitric acid (HNO3) oxidation giving carboxyl group (–CO2 H) generation on MWCNTs caps area in the aqueous media. The dispersion and, functionalizations were confirmed, respectively, through Atomic Force Microscopy (AFM) and, Fourier Transform Infra Red (FT-IR) absorption spectroscopy observations for carboxyl groups presence in the functionalized samples of MW-CNTs. The λ-DNA fragment's attachment reaction to the functionalized MW-CNTs cap regions over a 24 hours time-period at 4 °C furnished MW-CNT-λ-DNA bioconjugate which were confirmed by electrophoretic movement observations of the bioconjugate product's sample. It is the first report of chemical conjugation of supramolecular biological material, i.e., λ-DNA consisting of 48, 502 base pairs, to the carboxyl (–CO2H) functionalized MW-CNTs. The bioconjugate was found to be stable and, robust during preparation and, processing. The conjugated product could be used in designing prototypical DNA based biosensor for engagement in chemo-biological sensing and, as biomolecular transporter for various kinds of pay-load delivery purposes.
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Keywords: 48502 BASE-PAIR DNA FRAGMENT; CARBON NANOTUBE FUNCTIONALIZATION; DNA CONJUGATION; MULTI-WALLED CARBON NANOTUBES; MW-CNT-DNA BIOCONJUGATE; λ-DNA

Document Type: Research Article

Publication date: August 1, 2013

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  • Bionanoscience attempts to harness various functions of biological macromolecules and integrate them with engineering for technological applications. It is based on a bottom-up approach and encompasses structural biology, biomacromolecular engineering, material science, and engineering, extending the horizon of material science. The journal aims at publication of (i) Letters (ii) Reviews (3) Concepts (4) Rapid communications (5) Research papers (6) Book reviews (7) Conference announcements in the interface between chemistry, physics, biology, material science, and technology. The use of biological macromolecules as sensors, biomaterials, information storage devices, biomolecular arrays, molecular machines is significantly increasing. The traditional disciplines of chemistry, physics, and biology are overlapping and coalescing with nanoscale science and technology. Currently research in this area is scattered in different journals and this journal seeks to bring them under a single umbrella to ensure highest quality peer-reviewed research for rapid dissemination in areas that are in the forefront of science and technology which is witnessing phenomenal and accelerated growth.
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