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Study the Interaction of DNA with Halloysite Nanotube-Gold Nanoparticle Based Composite

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This study represents the formation of gold nanoparticles (AuNPs) and Halloysite nanotube based composites which can be acts as probe for the study of DNA damage. There are various methods have been developed for the study of DNA damage in various organisms. All of these methods have their own limitation that is either their complexity or tedious procedures. Here we have successfully reported adsorption of DNA on Halloysite nanotube supported Gold nanoparticles (AuNPs). Gold nanoparticles were synthesized by reduction of Chloroauric acid (HAuCl4) using Halloysites and Formation of immobilized gold nanoparticles on Halloysite was confirmed by FTIR and EDXRF. This gold immobilized on Halloysite nanotubes were used for the study of DNA adsorption by using spectrophotometry and spectrofluorometry. Usually UV absorption decrease from the conversion of DNA from double stranded to single stranded DNA form, but after being combined with gold nanoparticles absorption increase and fluorescence intensity decrease as subsequent increase in amount of single stranded DNA. Our study also proves that the synthesis of Halloysite nanotubes and gold nanoparticles based nanocomposites has advantages of both nanocomposite of Halloysite and gold nanoparticles. It may be widely applied biological as well as medical field.
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Document Type: Research Article

Publication date: December 1, 2012

More about this publication?
  • Bionanoscience attempts to harness various functions of biological macromolecules and integrate them with engineering for technological applications. It is based on a bottom-up approach and encompasses structural biology, biomacromolecular engineering, material science, and engineering, extending the horizon of material science. The journal aims at publication of (i) Letters (ii) Reviews (3) Concepts (4) Rapid communications (5) Research papers (6) Book reviews (7) Conference announcements in the interface between chemistry, physics, biology, material science, and technology. The use of biological macromolecules as sensors, biomaterials, information storage devices, biomolecular arrays, molecular machines is significantly increasing. The traditional disciplines of chemistry, physics, and biology are overlapping and coalescing with nanoscale science and technology. Currently research in this area is scattered in different journals and this journal seeks to bring them under a single umbrella to ensure highest quality peer-reviewed research for rapid dissemination in areas that are in the forefront of science and technology which is witnessing phenomenal and accelerated growth.
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