Bacterial Adsorption Onto Monolayer Ferromagnetic Nanofilms
In order to be able to optimize the decoration of bacteria with magnetic nanoparticles, we have developed a methodology for investigating the attachment of nanoparticles to the surface of bacteria by depositing a thin layer of the nanoparticles onto a Si(Ti)O2 waveguide surface
and measuring the bacterial (Rhodococcus erythropolis IGTS8) attachment kinetics under hydrodynamically controlled conditions. This is done by analysing the perturbation of the optical evanescent field generated by light guided along the substratum using optical waveguide lightmode
spectroscopy (OWLS), with which the number of deposited particles and attached bacteria could be precisely and accurately determined. Despite the strong attachment implied by the positive surface charge of the nanoparticles and the negatively charged surface of the bacteria, the bacteria are
initially attached reversibly. A criterion for ensuring that mixing of the nanoparticles and the bacteria is prolonged sufficiently for effective decoration to take place is proposed.
Keywords: ADSORPTION; BACTERIA; KINETICS; MAGNETIC NANOPARTICLES; OPTICAL WAVEGUIDE LIGHTMODE SPECTROSCOPY
Document Type: Research Article
Publication date: 01 June 2010
- Bionanoscience attempts to harness various functions of biological macromolecules and integrate them with engineering for technological applications. It is based on a bottom-up approach and encompasses structural biology, biomacromolecular engineering, material science, and engineering, extending the horizon of material science. The journal aims at publication of (i) Letters (ii) Reviews (3) Concepts (4) Rapid communications (5) Research papers (6) Book reviews (7) Conference announcements in the interface between chemistry, physics, biology, material science, and technology. The use of biological macromolecules as sensors, biomaterials, information storage devices, biomolecular arrays, molecular machines is significantly increasing. The traditional disciplines of chemistry, physics, and biology are overlapping and coalescing with nanoscale science and technology. Currently research in this area is scattered in different journals and this journal seeks to bring them under a single umbrella to ensure highest quality peer-reviewed research for rapid dissemination in areas that are in the forefront of science and technology which is witnessing phenomenal and accelerated growth.
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