Skip to main content
padlock icon - secure page this page is secure

Protein Detection at Zeptomole Quantities: A Nanoparticle Based Immunoassay Using Total Internal Reflection Fluorescence Microscopy

Buy Article:

$106.34 + tax (Refund Policy)

An immunoassay using total internal reflection fluorescence (TIRF) microscopy was developed to detect zeptomole quantities of protein. This approach was demonstrated using cancer antigen 125 (CA125), an ovarian cancer associated oncoprotein, as the analyte, and an anti-CA125 monoclonal antibody pair to capture and label the oncoprotein with a fluorescent nanoparticle. The captured and labeled analyte was imaged using TIRF microscopy as individual fluorescent binding events, enabling quantification of <1 zmol of CA125 with an intra-assay precision within ±10%. The low detection limit of this method demonstrates its potential utility as a tool for the proteomics of single cell protein quantities.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: NANOPARTICLE; PROTEIN ASSAY; SINGLE MOLECULE DETECTION; TIRF

Document Type: Short Communication

Publication date: December 1, 2008

More about this publication?
  • Bionanoscience attempts to harness various functions of biological macromolecules and integrate them with engineering for technological applications. It is based on a bottom-up approach and encompasses structural biology, biomacromolecular engineering, material science, and engineering, extending the horizon of material science. The journal aims at publication of (i) Letters (ii) Reviews (3) Concepts (4) Rapid communications (5) Research papers (6) Book reviews (7) Conference announcements in the interface between chemistry, physics, biology, material science, and technology. The use of biological macromolecules as sensors, biomaterials, information storage devices, biomolecular arrays, molecular machines is significantly increasing. The traditional disciplines of chemistry, physics, and biology are overlapping and coalescing with nanoscale science and technology. Currently research in this area is scattered in different journals and this journal seeks to bring them under a single umbrella to ensure highest quality peer-reviewed research for rapid dissemination in areas that are in the forefront of science and technology which is witnessing phenomenal and accelerated growth.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more