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Fluorescent Nanoparticles for Stimulated Emission Depletion Microscopy

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While most of the fluorescent nanoparticles used in stimulated emission depletion (STED) microscopy have a long excitation wavelength, many applications need shorter wavelength fluorophores, which are yet to be developed for STED microscopy applications. Here, three kinds of fluorescent nanoparticles, namely silica nanoparticles (NFv465), fluoro-max blue aqueous fluorescent nanoparticles (FBs) and light yellow nanoparticles (LYs) with short excitation wavelength in violet band have been studied to assess whether they are applicable in STED microscopy. The experimental configuration utilizes a 405 nm continuous wave (CW) laser as excitation beam and a 532 nm CW laser as depletion beam. We compare the photostability, photobleaching and depletion efficiency of three kinds of fluorescent nanoparticles in a series of experiments. Light yellow nanoparticles are proved to be a good candidate as fluorophore in STED microscopy.
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Keywords: Depletion Efficiency; Photobleaching; Stimulated Emission Depletion (STED) Microscopy

Document Type: Research Article

Affiliations: 1: Biomedical Engineering Program and Department of Mechanical Engineering, University of South Carolina, Columbia, SC 29208, USA 2: Institute of Photonics and PhotoPhoton-Technology, International Scientific and Technological Cooperation Base of Photoelectric Technology and Functional Materials and Application, Northwest University, Xi’an 710069, People’s Republic of China 3: AcZon S.r.l, Via Lavino, 265-D 40050 Monte San Pietro, Bologna, Italy 4: Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, USA

Publication date: April 1, 2020

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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