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Colorimetric Detection of Organophosphorus Pesticides Based on the Broad-Spectrum Aptamer

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Organophosphorus pesticides (OPs) are an important class of common pesticides. However, due to the abuse of organophosphorus pesticides, pesticide residue problems have been highlighted. In this study, the colorimetric detection method for OPs residues was established based on the broadspectrum aptamer. Through optimization, isocarbophos and omethoate were analyzed to verify the performance of the colorimetric assay. Under optimal conditions, the method proved effective for detecting an isocarbophos range of 50–1000 ng/mL and an omethoate range of 100–500 ng/mL. The protective effects of broad-spectrum aptamers and the split (28-mer P1+26-mer P2) aptamers on gold nanoparticles (AuNPs) were also comparatively studied. The present results suggested that the colorimetric assay based on broad-spectrum aptamer could be applied for a preliminary screening of different kinds of OPs.
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Keywords: Broad-Spectrum Aptamer; Colorimetric Detection; Gold Nanoparticles (AuNPs); Organophosphorus Pesticides

Document Type: Research Article

Affiliations: 1: Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun 130062, China 2: Experimental Teaching Center, College of Food Science and Engineering, Jilin University, Changchun 130062, China

Publication date: April 1, 2020

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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