In-Vitro Cytotoxicity and Oxidative Stress Induced by Cerium Aminoclay and Cerium Oxide Nanoparticles in Human Skin Keratinocyte Cells
Cerium oxide nanoparticles (CeO2 NPs) contain a number of properties suitable for biomedical, environmental and cosmetic applications, and in fact, they are well-known as inorganic sunscreen agents. Recently, cerium aminoclay (CeAC) has been considered as a hybrid material
for sunscreen application, however, the basic information on the toxicity and oxidative stress induced by CeAC and CeO2 NPs on cell lines remains scanty. Therefore, the present study performed an MTT assay and ROS measurement to assess the cell viability and oxidative stress on
HaCaT cells. The results showed that CeAC and CeO2 NPs exhibited low toxicities (IC50 values of 88.74/86.95 μg/mL and 84.13/83.13 μg/mL for 24 and 48 h, respectively). In particular, CeAC showed less toxicity than that did CeO2, due to its
larger size, resulting in less penetration into the cell membrane, which fact reduced the level of toxicity. Notably, the coexistence of Ce3+ and Ce4+ oxidation states has been shown to promote the antioxidant activity of Ce nanomaterials, playing a major role in enhancing
radical scavenging as well as reducing the intracellular ROS level on HaCaT cells. According to this estimates, CeAC and CeO2 NPs can be considered to be promising candidates for future cosmetic applications, especially sunscreens.
Keywords: Cerium Aminoclay; Cerium Oxide; HaCaT Cells; Human Skin Keratinocyte Cells; In-Vitro Cytotoxicity; Oxidative Stress
Document Type: Research Article
Affiliations: 1: Department of BioNano Technology, Gachon University, 1342 Seongnam-Daero, Sujeong-Gu, Seongnam-Si, Gyeonggi-do 13120, Republic of Korea 2: Myeong-dong Campus, Jeonghwa Arts College, 16-gil 21, Toegye-ro, Jung-gu, Seoul 04631, Republic of Korea
Publication date: 01 October 2019
- Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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