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Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks

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We report on a novel 3-dimensional (3D) network of crosslinked Cytochrome C/Carbon Nanotube (CytC/CNT) on a thiol-modified gold surface which can establish direct electrical communication between the redox center of Cytochrome C and the electrode. Cyclic voltammograms (CVs) results showed a pair of well defined redox peaks for Cytochrome C, located at about -0.03 and +0.06 V, cathodic and anodic respectively. Additionally, the formal potential E 0 of adsorbed Cyt c was found to be 15 mV, a value close to that of native Cyt c. Based on 3D Cytochrome c and carbon nanotube network, a sensitive superoxide radical biosensor has been proposed. The biosensor showed high sensitivity and lower detection limit of 0.3 μM of superoxide.
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Keywords: 3D Crosslinked Networks; Biosensor; Cytochrome C; Superoxide

Document Type: Research Article

Affiliations: 1: Faculty of Industrial Sciences and Technology, University Malaysia Pahang, Kuantan 26300, Malaysia 2: School of Chemistry, The University of Sydney, Sydney 2006, Australia

Publication date: August 1, 2017

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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