Anti-Inflammatory Bone Protective Effects of Nano-Protein Extracts from Mushroom Species: Ganoderma lucidum and Pleurotus ostreatus
Macrophages play an important role in mediating an inflammatory response by releasing inflammatory mediators such as interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and nitric oxide (NO). Excessive or unregulated secretion of these mediators will cause chronic inflammatory
diseases and harm bone growth necessary next to orthopedic implants to ensure device integration. The objective of the present in vitro study was to investigate the cytotoxicity of novel nano protein extracts from mushroom species on macrophages and healthy bone cells. Results of the
present study demonstrated for the first time that the nano protein extracts from the mushroom species Ganoderma lucidum and Pleurotus ostreatus can greatly suppress the proliferation of macrophages (85% and 75% decreases in macrophage density over a 4-day incubation time period
at a protein concentration as low as 0.125 mg/ml), thus potentially inhibiting harmful acute and chronic inflammation. Meanwhile, results showed that the nano protein extracts from the two mushroom species had no inhibitory behavior on the growth of healthy human osteoblast cells. Such results
lay the foundation for the functionalization of the nano protein extracts to nanoparticles for their delivery in the body to treat a wide range of orthopedic ailments. In conclusion, the results on this in vitro study provided significant promise for the further study of Ganoderma
lucidum and Pleurotus ostreatus where anti-inflammatory and pro-bone cell responses are necessary, such as those that immediately follow orthopedic implant insertion and numerous inflammatory orthopedic diseases.
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Document Type: Research Article
Department of Chemical Engineering, Northeastern University, Boston, Massachusetts, 02115, USA
Biotechnology Group, Natural Sciences Faculty, Universidad de Antioquia, Medellín, Antioquia, Colombia, Universidad de Antioquia, A.A. 1226
August 1, 2017
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