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Fabrication of CdTe Quantum Dots-Apoferritin Arrays for Detection of Melamine

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Melamine has been illegally adulterated in animal feeds and infant formulas to increase the content of crude protein, which results in outbreak of food borne illness such as kidney stones, and renal failure. Accordingly, it is important to detect melamine and its related compounds in foods. In the present work, a method for the detection of melamine by employing two-dimensional (2D) CdTe quantum dots (CdTe QDs)-apoferritin array prepared on modified silicon (Si) surface was proposed. CdTe QDs were synthesized in the cavity of horse spleen apoferritin (HsAFr). The characterization study showed that CdTe QDs were successfully synthesized with average diameters from 1.5 to 3 nm. In addition, when 2D (CdTe QDs)-apoferritin arrays were conjugated with melamine, a significant quenching of the fluorescence was observed. The fluorescence intensity was a linear function of the melamine concentrations up to 2 ppm with a correlation coefficient of 0.9879. Indeed, the CdTe QDs-apoferritin nanodot arrays can be employed as a useful sensing media for melamine detection.
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Keywords: Apoferritin; CdTe Quantum Dot; Fluorescence; Melamine

Document Type: Research Article

Affiliations: Department of Chemical and Biological Engineering, Gachon University, Seongnam 461-701, Korea

Publication date: August 1, 2017

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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