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Enhanced Enzymatic Activity of Laccase (from Pycnoporus sanguineus CS43) Immobilized on Sputtered Nanostructured Gold Thin Films

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Functionalization of thin films with organic ligands has been the subject of intense research due to their potential application as heterogeneous molecular nanosystems. In this work, self-assembled monolayers of thiols (16-mercaptohexadecanoic acid and 11-mercaptoundecanol) were used to bind laccase (from Pycnoporus sanguineus CS43) to nanostructured gold thin films obtained by DC sputtering. Sputtering power, sputtering pressure and substrate temperature were optimized to enhance the activity of the immobilized biomolecules. Scanning electron microscopy, confocal microscopy, X-ray diffraction and UV-vis spectroscopy were used to characterize the SAM-functionalized gold substrates. Our results demonstrate that the highest immobilized enzyme activity values can be achieved on substrates of surface roughness ˜200 nm and Au particle size of about 14 nm. The outstanding quality of the as-prepared substrates makes them particularly attractive as bionanosensors.
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Keywords: Enzyme Immobilization; Gold; Laccase; Nanostructured Thin Films

Document Type: Research Article

Affiliations: 1: Laboratorio de Nanotecnología Ambiental, Centro del Agua para América Latina y el Caribe, Instituto Tecnológico de Monterrey, 64849, Monterrey, Nuevo León, Mexico 2: Department of NanoEngineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA 3: Laboratorio de Enzimología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León. Av. Universidad, Ciudad Universitaria, 64450, San Nicolás de los Garza, Nuevo León, Mexico 4: Centro de Investigación en Materiales Avanzados, Unidad Monterrey, 66600, Apodaca, Nuevo León, Mexico

Publication date: February 1, 2017

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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