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In Vitro Evaluation of Carbon Nanotube-Based Scaffolds for Cartilage Tissue Engineering

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Cartilage injuries are one of the most common musculoskeletal disorders. Although a number of techniques have been designed to treat cartilage lesions, current research is focused on tissue engineering methods. Recent advances in molecular biology, biotechnology, and polymer science have led to both the experimental and clinical application of various cell types and biomaterials in the treatment of cartilage injuries. This study reports an exceptionally good growth of chondrocytes on a 3D scaffold, based on multiwalled carbon nanotubes (MWCNTs). The MWCNT-based nanostructural scaffold was fabricated employing a plasma enhanced chemical vapor deposition technique. Articular cartilage specimens were taken from the distal femur of adolescent New Zealand White rabbits and chondrocytes were obtained by collagenase digestion. The scaffolds were characterized using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). Cell-seeded constructs were visualized with SEM. Chondrocytes adhered to the MWCNT surfaces and were evenly distributed. After one week of culturing, the number of cells was tripled on the MWCNT scaffolds in respect to the culturing in a plastic flask. These cells displayed multiple cytoplasmic extensions that interact with the MWCNTs. The nanotubes were bent by the cells and the cell morphology was altered through the nanostructured surface. Our study confirms that MWCNT-based surfaces provide a very good scaffold that can be used to stimulate the formation of cartilage tissue.
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Keywords: Cartilage Tissue Engineering; Chondrocyte; Multi Walled Carbon Nanotubes; Scaffold

Document Type: Research Article

Affiliations: 1: Department of Orthopedics and Traumatology, Poznan University of Medical Sciences, 135-147, 28 czerwca 1965 St, 61-545 Poznan, Poland 2: Faculty of Chemistry, Adam Mickiewicz University, 61-614 Poznań, Poland 3: Department of Physics, Freie Universit├Ąt Berlin, 14195 Berlin, Germany

Publication date: September 1, 2016

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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