In this article, a surface plasmon resonance (SPR) biosensor based on graphene oxide (GO) for simple and rapid determination of D-dimer in plasma is introduced. GO was conjugated onto the surface of the SPR chip surface via electrostatic interaction after the chip surface was activated with cysteamine. Subsequently, anti-D-dimer was covalently immobilized on the chip surface through binding between carboxylic groups on the GO surface and amine groups on the protein surface. Finally, label-free and rapid detection of D-dimer was carried out by the SPR biosensor via protein–protein interaction. After optimization, the concentration of GO was found to be 2 mg mL−1. Meanwhile, this biosensor presented a response to D-dimer in the concentration range of 10–150 ng mL−1, and limit of detection (LOD) of the developed biosensor was determined to be as low as 5.08 ng · mL−1. In addition, addition of albumin from human serum (HSA) to the anti-D-dimer-modified SPR chip revealed a negligible response, indicating a satisfactory immunological specificity. Furthermore, D-dimer detection was successfully conducted in different concentrations of human plasma. These results suggest that the GO-assisted SPR biosensor could be exploited for point-of-care diagnosis in the clinical laboratory in the future.
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Surface Plasmon Resonance
Document Type: Research Article
School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan 430065, China; Department of Clinical Laboratory, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, China
Wuhan Thalys Medical Care Ltd., 1310 Jinshan Avenue, Dongxihu District, Wuhan 430040, China
School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan 430065, China
July 1, 2016
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