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Graphene Oxide-Assisted Surface Plasmon Resonance Biosensor for Simple and Rapid Determination of D-Dimer in Plasma

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In this article, a surface plasmon resonance (SPR) biosensor based on graphene oxide (GO) for simple and rapid determination of D-dimer in plasma is introduced. GO was conjugated onto the surface of the SPR chip surface via electrostatic interaction after the chip surface was activated with cysteamine. Subsequently, anti-D-dimer was covalently immobilized on the chip surface through binding between carboxylic groups on the GO surface and amine groups on the protein surface. Finally, label-free and rapid detection of D-dimer was carried out by the SPR biosensor via protein–protein interaction. After optimization, the concentration of GO was found to be 2 mg mL−1. Meanwhile, this biosensor presented a response to D-dimer in the concentration range of 10–150 ng mL−1, and limit of detection (LOD) of the developed biosensor was determined to be as low as 5.08 ng ยท mL−1. In addition, addition of albumin from human serum (HSA) to the anti-D-dimer-modified SPR chip revealed a negligible response, indicating a satisfactory immunological specificity. Furthermore, D-dimer detection was successfully conducted in different concentrations of human plasma. These results suggest that the GO-assisted SPR biosensor could be exploited for point-of-care diagnosis in the clinical laboratory in the future.

Keywords: Biosensor; D-Dimer; Detection; Graphene Oxide; Plasma; Surface Plasmon Resonance

Document Type: Research Article

Affiliations: 1: School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan 430065, China; Department of Clinical Laboratory, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, China 2: Wuhan Thalys Medical Care Ltd., 1310 Jinshan Avenue, Dongxihu District, Wuhan 430040, China 3: School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan 430065, China

Publication date: July 1, 2016

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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