Graphene Oxide Based Fluorometric Detection of Hydrogen Peroxide in Milk

We report a highly rapid, visual, precise, selective and sensitive analytical method for the determination of hydrogen peroxide (H₂O₂) in milk using Graphene oxide (GO) with 2′,7′-dichlorfluorescein diacetate (DCFH-DA). A 1000 μL aliquots of 10-fold diluted samples (high and low-fat milk) directly onto the 100 μL of GO and 100 μL of 100 μM DCFH-DA produced green colour under Ultraviolet light at 365 nm. The analytical feature of our proposed method includes low detection limit (10 mmol mL⁻¹) and satisfactory recovery values for samples. The presence of H₂O₂ in milk is a major concern because it constitutes a public health hazard. Many milk indursties are using H₂O₂ as a preservative, but if the concentration increases then it causes so many health problems such as neurodegenerative disorders, cancer and diabetes. Present methods show an easy way for detecting H₂O₂ generally require considerable time and laboratory facilities. The chemical tests have sufficient sensitivity to detect wide linear range of H₂O₂ concentration.