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Immobilization and Characterization of Penicillin G Acylase (PGA) Immobilized on Magnetic Ni0.5Zn0.5Fe2O4 Nanoparticles

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Magnetic Ni0.5Zn0.5Fe2O4 nanoparticles were prepared via the solution combustion process and their microstructure and magnetic properties were investigated by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM). The as-prepared magnetic Ni0.5Zn0.5Fe2O4 nanoparticles are characterized with average grain size of about 20 nm and magnetization of 90.3 Am2/kg. The surface of magnetic Ni0.5Zn0.5Fe2O4 nanoparticles was modified by use of sodium silicate and N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, and the penicillin G acylase (PGA) was successfully immobilized on the surface-modified magnetic Ni0.5Zn0.5Fe2O4 nanoparticles. The results show that the activity for the immobilized PGA is affected less by pH and temperature than that for the free PGA, and the immobilized PGA exhibits a high effective activity, good stability of enzyme catalyst. This immobilized PGA on magnetic Ni0.5Zn0.5Fe2O4 nanoparticles can be separated from the solution by the external magnetic field for cyclic utilization, and they could retain about 70% of initial enzyme activity after 11 consecutive operations. The kinetic parameters (K m and v max) were determined, and the value of K m for the immobilized PGA (204.53 mmol/L) is higher than that of the free enzyme (3.50 mmol/L), while v max (1.93 mmol/min) is also larger than that of the free enzyme (0.838 mmol/min).
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Document Type: Research Article

Publication date: January 1, 2016

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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