A Novel Electrochemical Genosensor Based on Banana and Nano-Gold Modified Electrode Using Tyrosinase Enzyme as Indicator
The electrochemical behavior of the tyrosinase enzyme at the surface of two electrodes, carbon paste electrode (CPE) and nano-gold modified carbon paste electrode (NGCPE), has been studied by cyclic voltammetry. Tyrosinase showed one oxidation peak (around +0.85 V) and one reduction
peak at +0.40 V versus Ag|AgCl|KCl (3 M). To calculate the values of α and ks
, the effect of potential scan rate on the electrochemical properties of tyrosinase was investigated. Cyclic voltammetry and UV-vis absorption techniques were used for the study of interaction
between DNA and tyrosinase. The cyclic voltammogram of tyrosinase was obtained in the presence of different types of DNA bases for the study of tyrosinase-DNA binding. The results showed that the hydrogen binding and electrostatic interactions were important interaction mode. Moreover, a variation
in tyrosinase signals intensity regarding the interaction to ssDNA and dsDNA was observed. The selectivity of the biosensor was studied using noncomplementary oligonucleotides. Finally, banana modified carbon paste electrode was also prepared to investigate the interaction of banana's tyrosinase
with DNA. The limit of detection for DNA probe was calculated 0.33 pM by using the oxidation signal of accumulated tyrosinase in NGCPE.
Document Type: Research Article
Publication date: 01 May 2015
- Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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