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Scanning Electron Microscopy Study of the Effect of the Brushing Time on the Human Tooth Dentin After Exposure to Acidic Softdrinks

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Scanning electron microscopy (SEM) was used to examine the abrasive and erosive potential of the brushing time on the dentin surface eroded by acidic soft drinks to suggest an optimized toothbrushing start time after the consumption of cola (pH 2.52) in children. Thirty-six non-carious primary central incisors were assigned to 12 experimental groups (n =3) based on the erosive and abrasive treatment protocols. Cola exposure was used as the erosive treatment. Three brushing durations (5, 15, and 30 sec) and four brushing start times (immediately, 30 min, 60 min, and 120 min) after an erosive pre-treatment were used for the abrasive treatment. Toothbrushing after exposure to acidic soft drinks led to an increase in the open-tubule fraction and microstructural changes. Toothbrushing immediately after the erosive pre-treatment showed the largest abrasive and erosive potential on the dentin whereas that 60 and 120 min after the pre-treatment showed the least abrasive and erosive potential on the dentin. Toothbrushing for both 60 and 120 min after the pre-treatment showed similar erosive and abrasive potentials on the dentin. The brushing duration showed no effect on the erosive and abrasive potential on the dentin. Therefore, to achieve the desired tooth surface cleaning and less surface lesion on the dentin surface, toothbrushing should be performed at least 1 hour after cola consumption. Three-minute brushing after cola consumption is sufficient to prevent dental lesions, and prolonged brushing can irritate the gingival tissues.
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Document Type: Research Article

Publication date: July 1, 2012

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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