Immobilization of Tyrosinase in Carboxylic and Carbonyl Group-Modified MWNT Electrode and Its Application for Sensing Phenolics in Red Wines
Tyrosinase-immobilized biosensor was fabricated based on PAAc-g-MWNT and PMAn-g-MWNT, respectively. The poly(acrylic acid)-grafted multi-wall carbon nanotubes, PAAc-g-MWNT, and poly(maleic anhydride)-grafted multi-wall carbon nanotube, PMAn-g-MWNT, were prepared by radiation-induced
graft polymerization of acrylic acid (AAc) and maleic anhydride (MAn) on the surface of MWNT. The biosensor was prepared on ITO glass electrode by coating of chitosan solution with tyrosinase-immobilized PAAc-g-MWNT and PMAn-g-MWNT, respectively. The sensing ranges of the tyrosinase-immobilized
biosensor based on PAAc-g-MWNT and PMAn were in the range of 0.2–0.9 mM concentration and in the range of 0.1–0.5 mM for phenol in phosphate buffer solution, respectively. Optimal pH and temperature conditions for sensing various phenolic compounds with tyrosinase-immobilized biosensor
were determined. Total phenolic content for three commercial red wines on tyrosinase-immobilized biosensor were also determined.
Keywords: PHENOLICS; RADIATION-INDUCED GRAFT POLYMERIZATION; RED WINES; TYROSINASE-IMMOBILIZED BIOSENSOR
Document Type: Research Article
Publication date: 01 June 2010
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