The Immunotherapeutic Effect of Fe₃O₄ Nanoparticles as Adjuvants on Mice H₂₂ Live Cancer

The aim of this paper is to prepare Fe₃O₄ nanoparticles and study its immunotherapeutic effect as adjuvants on mice H₂₂ live cancer. The Fe₃O₄ nanoparticles were prepared by chemical coprecipitation route. Transmission electron microscopy (TEM), X-ray diffraction (XRD), Energy Dispersive Analysis (EDS) were used to characterize Fe₃O₄ nanoparticles. The Fe₃O₄ nanoparticles were compared with the common alum adjuvants for its ability to induce immunity to inhibit tumor growth rate by prophylactic and therapeutic studies. Results indicated that Fe₃O₄ nanopaticles adsorbed autovaccine took great advantages over the common alum adjuvants after subcutaneous injection, raised the mass inhibitory rate of tumor, boosted the activity of cytotoxicity and enhanced the level of IFN-γ cytokine. Thus, we concluded that Fe₃O₄ nanoparticles as adjuvants had great potential for enhancing anti-tumor immune response.