Skip to main content
padlock icon - secure page this page is secure

Bio-Detection of Dipeptides by the ZrO2/PVA/Cyt.c Multilayer Film

Buy Article:

$106.39 + tax (Refund Policy)

The adsorption mechanism of dipeptides namely Gly-Glu, Asp-Glu with protein layer was successfully examined on cytochrome c (Cyt.c) surface probing by fluorescent dye molecule. Molecularly thin Cyt.c layer was immobilized on poly(vinyl alcohol) (PVA) coated zirconium oxide (ZrO2) gel films by electrostatic interaction. Denaturation of Cyt.c was minimized on sol–gel derived ZrO2-gel film by PVA. The conformation of immobilized protein was verified by FTIR-ATR measurements. The fluorescent dye molecule was easily bound on the Cyt.c layer. The dye adsorbed protein thin film was soaked in two different dipeptides solution with various concentrations. The adsorption of dipeptides on Cyt.c thin film and consequent desorption of fluorescent dye from Cyt.c layer in dipeptide solutions was monitored by UV-vis absorption, fluorescence spectroscopy and quartz crystal microbalance method. The ion exchange mechanism on protein surface between fluorescent dye and dipeptides is thought to be responsible for dye desorption and formation of relatively strong electrostatic bonding between dipeptides with protein. The adsorption behavior curve of dipeptides was analyzed by Hill equation. The number of functional group together with size of dipeptides play an important role for different adsorption parameters obtained from this model.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: HILL EQUATION; ION EXCHANGE; METAL OXIDE THIN FILM; PROTEIN IMMOBILIZATION; SOL-GEL PROCESS

Document Type: Research Article

Publication date: June 1, 2009

More about this publication?
  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more