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Bio-Detection of Dipeptides by the ZrO2/PVA/Cyt.c Multilayer Film

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The adsorption mechanism of dipeptides namely Gly-Glu, Asp-Glu with protein layer was successfully examined on cytochrome c (Cyt.c) surface probing by fluorescent dye molecule. Molecularly thin Cyt.c layer was immobilized on poly(vinyl alcohol) (PVA) coated zirconium oxide (ZrO2) gel films by electrostatic interaction. Denaturation of Cyt.c was minimized on sol–gel derived ZrO2-gel film by PVA. The conformation of immobilized protein was verified by FTIR-ATR measurements. The fluorescent dye molecule was easily bound on the Cyt.c layer. The dye adsorbed protein thin film was soaked in two different dipeptides solution with various concentrations. The adsorption of dipeptides on Cyt.c thin film and consequent desorption of fluorescent dye from Cyt.c layer in dipeptide solutions was monitored by UV-vis absorption, fluorescence spectroscopy and quartz crystal microbalance method. The ion exchange mechanism on protein surface between fluorescent dye and dipeptides is thought to be responsible for dye desorption and formation of relatively strong electrostatic bonding between dipeptides with protein. The adsorption behavior curve of dipeptides was analyzed by Hill equation. The number of functional group together with size of dipeptides play an important role for different adsorption parameters obtained from this model.
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Document Type: Research Article

Publication date: June 1, 2009

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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