The Influence of Growth Regulators on In-Vitro Culture of Rosa Hybrida

Flowers affect people’s quality of life owing to their outstanding natural beauty. Among flowers, the beauty and the richness of color and smell make Roses of great importance. The micro propagation of Rosa Hybrida was done using apical and lateral buds to determine the optimum concentration for growth regulators in order to achieve the highest proliferation and rooting. In this research, the basal medium of Murashige and Skoog (MS) was applied in all stages. At the proliferation stage, six different levels (0, 0.1, 0.5, 1, 1.5 and 2 mg/l) of Benzyl Adenine (BA) as well as two different levels (0 and 0.01 mg/l) of Indolebutyric Acid (IBA) and at the rooting stage, different levels (0, 0.5, 0.75, 1, 2 and 3 mg/l) of IBA were used. The results showed that the maximum propagation of Rosa Hybrida was at 2 mg/l concentration of BA, the highest elongation, obtained at 0.5 mg/l BA and the optimal rooting occurred at 0.5 mg/l concentration of IBA. In addition, according to the research findings, IBA had no significant effect on the vegetative growth of the explants of Rosa Hybrida.