The Quality Test of White Rat Spermatozoa (Rattus norvegicus) Strain Sprague-Dawley by Given of Black Nightshade Juice (Solanum nigrum L.)

This study aims to determine the quality of the white rat spermatozoa by giving of black nightshade juice with the method of Complete Randomized Design. A total of 24 samples consisting of the male, white rat which criteria 2–3 months old, 200–300 g weight. Black nightshade juice that has given with a concentration of 40%, 50%, and 60% with a total of 3 millilitres per white rat per day for 28 days to every treatment group. Given black nightshade juice can decrease the quality of white rat spermatozoa. The result showed that black nightshade juice was able to reduce the percentage of motility of spermatozoa with a value of 47.5%±16.96. It can also reduce the concentration of spermatozoa up to 418.75 (106/mL)±62.62 and it can increase abnormalities of spermatozoa up to 24.833%±3.31. All of the parameters that have been tested and showed that optimal decrease in spermatozoa quality was in the concentration of 50% black nightshade juice.