Chemometric Methods for the Evaluation of Electron Transfer Properties of Zinc Oxide Nanorods Modified Gold Electrode for Lactate Detection in Food Products

Estimating lactate concentration in the meat food products has been a serious concern since it induces hepatic immaturity in children below the age of 3. This article is aimed to illustrate the statistical modeling for the estimation of lactate in meat food products and to identify the interrelation between the amount of lactate added to the phosphate buffered saline (0.1 M, 7.4 pH) and other electrochemical parameters such as amount of charge consumed, surface coverage, cathodic peak current, cathodic peak potential, full width at half maximum of cathodic peak height and electron transfer rate constant. The gold working electrode was modified by immobilizing lactate dehydrogenase on ZnO nanorods. The modified gold working electrode was used for the determination of lactate by linear sweep voltammetry. Multivariate data analysis was performed with the values of electrochemical parameters. This analysis showed the significant relation between the amount of charge consumed and full width at half maximum of cathodic peak height with the added lactate concentrations. Based on these relationships, a multiple linear regression model was framed for the estimation of lactate in meat food products.