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Selective Enrichment of Biomolecules Containing Cis-Diols Using Boronic Acid-Modified Gold Nanoparticles for Capillary Electrophoresis Analysis

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In this study, a selective enrichment method is developed for fast and efficient biomolecules containing cis-diols investigation. Gold nanoparticles were first synthesised, then modified with 4-mercaptophenylboronic acid to provide porous substrate with large specific surface and dual functions. Catechol was employed as a standard biomolecules to investigate the enrichment efficiency. In this way, the enrichment, washing and detection steps can all be investigated. Under the optimized conditions, the sensitivity was enhanced by 20-fold as compared with regular CE. With the established method, catechol in a human urine sample was determined.
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Document Type: Research Article

Publication date: September 1, 2012

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  • Journal of Computational and Theoretical Nanoscience is an international peer-reviewed journal with a wide-ranging coverage, consolidates research activities in all aspects of computational and theoretical nanoscience into a single reference source. This journal offers scientists and engineers peer-reviewed research papers in all aspects of computational and theoretical nanoscience and nanotechnology in chemistry, physics, materials science, engineering and biology to publish original full papers and timely state-of-the-art reviews and short communications encompassing the fundamental and applied research.
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