Effects of Graft Shell Thickness and Compositions on Lectin Recognition of Glycoparticles
Polystyrene particles bearing iniferter groups, obtained by an emulsifier-free emulsion polymerization, were subjected to photoiniferter polymerization using a glycomonomer, i.e., 4-vinylbenzenesulfonamidoethyl 1-thio-β-D-lactoside (glycomonomer 1 ). The glycopolymer-grafted polystyrene particles obtained were shown to have hydrated graft layers, 17–200 nm in thickness, on their 435-nm diameter cores. Particles bearing di-block copolymer shells were also prepared using glycomonomer 1 and polyethylene glycol monomethacrylate (PEG monomer 2 ) as the monomers. The thicknesses of the shell layers were controlled by the monomer concentrations and polymerization times (photo-irradiation times). The amount of lactose conjugated per unit of the core–shell particles was measured by the anthrone-sulfuric acid method, which was found to increase with the glycopolymer shell layer thickness. We also investigated the effects of grafted-glycopolymer shell thicknesses and their compositions on the adsorption of a specific lectin (peanut agglutinin (PNA)). For instance, the obtained particles were found to coagulate in the presence of PNA, indicating a highly specific response toward lectins. Furthermore, the particles bearing di-block copolymers were shown to prevent non-specific adsorption of the proteins effectively due to the PEG moieties on the inner positions of the shell layers.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
Document Type: Research Article
Publication date: March 1, 2013
More about this publication?
- Journal of Colloid Science and Biotechnology is an international multidisciplinary peer- reviewed journal covering all aspects of colloid science including colloids preparation, characterization, structure-property relationships, spectroscopy, chemical and physical properties and applications of colloids in biotechnology, medicine and pharmaceuticals. This journal deals with interdisciplinary research areas of chemistry, physics, biology, materials science, polymer science, nanotechnology covering all topics related to colloids such as latexes, emulsions, suspensions, micellar systems, gels, composites, hybrids, surfactants, aerosols, foams, minerals, soft matter, microfluidics, encapsulation processes of active molecules, biomolecules, surfacial and interfacial processes, catalysis, electrochemistry, theoretical aspects, computer simulations and colloids in biotechnology and medicine.
- Editorial Board
- Information for Authors
- Subscribe to this Title
- Ingenta Connect is not responsible for the content or availability of external websites