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The Use of Magnetic Poly(N-isopropylacrylamide) Microspheres for Separation of DNA from Probiotic Dairy Products

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Magnetic thermosensitive poly(N-isopropylacrylamide) (PNIPAAm) microspheres 3.6 μm in size were prepared by the inverse emulsion polymerization in paraffin oil in the presence of maghemite (γ-Fe2O3 nanoparticles. The polymerization was initiated with 2,2′-azobis(2-methyloctanenitrile) and emulsified with Span 80. γ-Fe2O3 nanoparticles (∼11 nm) were obtained by coprecipitation of Fe(II) and Fe(III) chlorides in a basic solution followed by oxidation with NaClO. The size, polydispersity and iron content of the magnetic PNIPAAm microspheres were characterized by light microscopy, transmission electron microscopy (TEM) and atomic absorption spectroscopy (AAS). The PNIPAAm microspheres contained 7.1 wt% of Fe (i.e., 11.1 wt% γ-Fe2O3 which was sufficient for them to be well attracted by a magnet. Thermosensitivity of water-swollen magnetic PNIPAAm microparticles was investigated by light microscopy equipped with a heating stage. Magnetic PNIPAAm microspheres were used for separation of DNA from probiotic dairy products (yoghurt) at different temperatures. Magnetic PNIPAAm microspheres proved to be suitable for quick and convenient temperature-controlled isolation of DNA from complex food samples.
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Keywords: DIARY PRODUCTS; DNA; MAGNETIC PARTICLES; N-ISOPROPYLACRYLAMIDE; SEPARATION; THERMOSENSITIVE

Document Type: Research Article

Publication date: December 1, 2012

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  • Journal of Colloid Science and Biotechnology is an international multidisciplinary peer- reviewed journal covering all aspects of colloid science including colloids preparation, characterization, structure-property relationships, spectroscopy, chemical and physical properties and applications of colloids in biotechnology, medicine and pharmaceuticals. This journal deals with interdisciplinary research areas of chemistry, physics, biology, materials science, polymer science, nanotechnology covering all topics related to colloids such as latexes, emulsions, suspensions, micellar systems, gels, composites, hybrids, surfactants, aerosols, foams, minerals, soft matter, microfluidics, encapsulation processes of active molecules, biomolecules, surfacial and interfacial processes, catalysis, electrochemistry, theoretical aspects, computer simulations and colloids in biotechnology and medicine.
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