Covalent Immobilization of Penicillin G Acylase onto Grafted Alginate Beads
Due to its large importance as the raw material for 65% of the world total semi-synthesis antibiotics market, 6-Amino penicillanic acid (6-APA) was recently manufactured enzymaticaly using the immobilization technique. In this work we used grafted alginate prepared according to our patent and using the Encapsulator to prepare it in uniform beads shape for covalent immobilizing penicillin G acylase (PGA). Four factors were studied to optimize the loading of PGA and the enzyme's loading capacity was increased from 1.7 U/g gel beads to up to 104 U/g gel. Some of the most interesting results was the effect of varying the buffer ionic strength as it showed an improvement of the enzyme loading capacity from 70 to 104 U/g gel beads (>30%) using a buffer concentration of 50 mM. However, it was found that low concentration of the buffer has a negative effect on the immobilized enzyme's pH stability at low pH range of 3–5. Furthermore, the immobilized enzyme was used for 15 times with retention of ∼80% of the enzyme's initial activity. In conclusion, the grafted alginate beads has proved to be a suitable natural, low priced and reproducible matrix for immobilizing and improving the efficiency and durability of the immobilized enzyme.
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Document Type: Research Article
Publication date: December 1, 2012
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- Journal of Colloid Science and Biotechnology is an international multidisciplinary peer- reviewed journal covering all aspects of colloid science including colloids preparation, characterization, structure-property relationships, spectroscopy, chemical and physical properties and applications of colloids in biotechnology, medicine and pharmaceuticals. This journal deals with interdisciplinary research areas of chemistry, physics, biology, materials science, polymer science, nanotechnology covering all topics related to colloids such as latexes, emulsions, suspensions, micellar systems, gels, composites, hybrids, surfactants, aerosols, foams, minerals, soft matter, microfluidics, encapsulation processes of active molecules, biomolecules, surfacial and interfacial processes, catalysis, electrochemistry, theoretical aspects, computer simulations and colloids in biotechnology and medicine.
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