Ethanol Production from Pretreated Diluted Acid Recycled Paper Sludge Using SHF and SSF
The objectives of this study were to produce ethanol from pretreated recycled paper sludge by separated hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) with a monoculture of Saccharomyces cerevisiae KM 5221 and Kluyveromyces marxinus 5057 and a co-culture ofS. cerevisiae KM 5221 and K. marxinus 5057 was studied. Recycled paper sludge was dried and milled into small pieces by blender, ultracentrifugal mill and disc mill and pretreated at 121 °C under vapor pressure of 15 lb/inch2 for 30 min with 0.1 M sulfuric acid. In hydrolysate of pretreated recycled paper sludge, the reducing sugar and glucose concentration were 10.08 g/L and 5.41 g/L and enzymatic digestibility of 66.67% cellulase loading 30,000 CMC Unit/g. In SHF, the ethanol production by K. marxinus 5057 and S. cerevisiae KM 5221 and were 14.52 g/L and 13.42 g/L, respectively, while in SSF, the ethanol concentrations were 7.94 g/L and 7.34 g/L, respectively. In SHF, the inoculums ration of 3 × 102 CFU/mL : 4 × 104 CFU/mL of S. cerevisiae KM 5221 and K. marxinus 5057, respectively, produced ethanol concentration (16.06 g/L) higher than that of the inoculums ration S. cerevisiae KM 5221 of 4 × 103 CFU/mL and K. marxinus 5057of 4 × 103 CFU/mL and monoculture while in SSF, the ethanol concentration of co-culture ratio 3 × 102 CFU/mL : 4× 104 CFU/mL of S. cerevisiae KM 5221 and K. marxinus 5057, respectively, was lower than that of in SHF. The results indicated that each strain of yeast was able to produce ethanol from recycled paper sludge and the co-culture produce greater amounts of ethanol and higher yields than that fermented by monoculture.
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Document Type: Research Article
Publication date: June 1, 2013
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