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Induction of Lubricin by Kartogenin and Growth Factors in Three-Dimensional Hydrogels

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Osteoarthritis (OA) is one of the main causes of disability in aging population and the effective therapy remains elusive. OA involves quantitative and/or qualitative alterations of lubricin, which is an essential mucinous glycoprotein secreted by synovial fibroblasts and chondrocytes. In order to increase the expression of lubricin in cartilage tissue engineering, we explored the effect of kartogenin (KGN), transforming growth factor-β1 (TGF-β1) and morphogenetic protein-7 (BMP-7) on the expression of lubricin in bone-derived mesenchymal stem cells (BMSCs) encapsulated in the poly(ethylene glycol) diacrylate (PEGDA) hydrogels. BMSCs or chondrocytes were incorporated into PEGDA hydrogels and cultured in an appropriate microenvironment fabricated with KGN, TGF-β1, and BMP-7 to detect the formed substance of cartilage. The results showed that BMSCs and chondrocytes could normally proliferate in the PEGDA hydrogels. The BMSCs-PEGDA constructs were treated with TGF-β1, BMP-7, and KGN and achieved higher lubricin expression and more extracellular matrices production, such as characteristic glycosaminoglycans (GAGs), compared with other groups. These results suggested that combination of TGF-β1, BMP-7, and KGN could enhance the level of lubricin significantly in BMSCs-based therapies, which may be a new method for the treatment of joint diseases. This study provides a research model and theoretical basis for the future cartilage regeneration medicine and related clinical trials.
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Keywords: BONE MORPHOGENETIC PROTEIN-7; CARTILAGE TISSUE ENGINEERING; HYDROGEL; KARTOGENIN; LUBRUCIN; TRANSFORMING GROWTH FACTOR-β1

Document Type: Research Article

Publication date: April 1, 2019

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  • Journal of Biomaterials and Tissue Engineering (JBT) is an international peer-reviewed journal that covers all aspects of biomaterials, tissue engineering and regenerative medicine. The journal focuses on the broad spectrum of research topics including all types of biomaterials, their properties, bioimplants and medical devices, biofilms, bioimaging, BioMEMS/NEMS, biosensors, fibers, tissue scaffolds, tissue engineering and modeling, artificial organs, tissue interfaces, interactions between biomaterials, blood, cells, tissues, and organs, regenerative medicine and clinical performance.
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