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Fat Mass and Obesity Gene was Potentially Related with Diabetic Retinopathy Through Immune Mechanism

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The role of inflammation in the development and progression of diabetic retinopathy (DR) has been studied for a long time, but in the last 10 years there has been enormous interest in the molecular mechanisms to develop novel therapeutic approaches. Of this study, there were none of significantly differences of Age, Sex, Body mass index, TC, TG and CRP in different groups. However, FPG values and HbA1c values in patients with/without DR were significantly higher than that in healthy group. Meanwhile, TNF-α contents in patients with DR were also significantly higher than that in patients without DR group. Over-expressed FTO gene in HepG cell resulted in a significant increase in mRNA and protein expression of TNF-α compared with that in wild group. Knockdown FTO gene in HepG cell had significantly reduce TNF-α expression compared with Control group, which indicated that knock-down FTO gene in HepG cell had significantly effects on TNF-α expression. Of the RNA dot-blot analysis, over-expressed FTO gene in HepG cell significantly promoted m6A expression. Meanwhile, knockdown FTO gene in HepG cell had significantly reduce m6A expression compared with Control group. The results revealed that knockdown and over-expressed FTO gene in HepG cell could effectively affect the m6A expression, which indicated the positive relationships between FTO gene and m6A in vitro. In addition, Retinal vessel in STZ-induced mice resulted in a significantly increase in mRNA and protein expression of TNF-α compared with that in wild group, which indicated that STZ-treated mice significantly promoted TNF-α expression. The results mentioned above revealed that STZ-induced treatment could effectively affect the m6A and TNF-α expression, which indicated the positive relationships between FTO gene and m6A in vivo. Characterization of the role of FTO gene in the development of DR should lead to a better understanding.
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Keywords: DIABETIC RETINOPATHY; FPG; FTO; STZ-TREATED MICE; TNF-α

Document Type: Research Article

Publication date: January 1, 2018

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  • Journal of Biomaterials and Tissue Engineering (JBT) is an international peer-reviewed journal that covers all aspects of biomaterials, tissue engineering and regenerative medicine. The journal focuses on the broad spectrum of research topics including all types of biomaterials, their properties, bioimplants and medical devices, biofilms, bioimaging, BioMEMS/NEMS, biosensors, fibers, tissue scaffolds, tissue engineering and modeling, artificial organs, tissue interfaces, interactions between biomaterials, blood, cells, tissues, and organs, regenerative medicine and clinical performance.
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